Intra Cytoplasmic Sperm Injection (ICSI)
What is ICSI?
Intracytoplasmic Sperm Injection or ICSI is a step ahead in the IVF treatment technique, where a single sperm is injected in the centre of a single mature egg (oocyte) cytoplasm with a very ﬁne needle called micropipette. ICSI has transformed male infertility in the past couple of years by helping men with poor quality of sperm and introducing them to fatherhood.
Who needs ICSI?
We use ICSI in conjunction with an IVF cycle when we believe that fertilization is unlikely to occur using only conventional IVF. Most typically, ICSI is appropriate when there is a malefactor to your infertility. We might recommend ICSI when:
- The number of sperm is low
- There’s poor sperm motility (movement)
- There’s a high number of abnormal sperm
- The sperm has been collected surgically
- There are high levels of antibodies in the semen
- There have been previous unexplained failures to achieve fertilization in conventional IVF, or when very few eggs have fertilized following IVF
- Sperm function tests have shown that the sperm would be unlikely to achieve fertilization, or embryo quality and implantation may be compromised.
1 )Ovarian Stimulation
After primary consultation with our fertility experts, the process begins with stimulating the ovaries from Day 2 or Day 3 of the menstrual cycle. In this step, scanning of follicles is done, and daily hCG injections are given for 10-12 days to the female partner to grow the follicles and our fertility expert monitors the development of the follicles in the ovary using an ultrasound and blood investigations on regular interval to control the size and quality of follicles.
2 ) Transvaginal Oocyte Retrieval
After 10-12 days of stimulation based on the growth an injection (trigger) is given to assist the final maturation of the eggs and loosening the egg from the walls of follicle. After 34-36 hours of trigger injection, the egg retrieval process takes place with general anesthesia in the IVF unit of the Zivia Fertility centre.
3 ) Sperm collection
On the same day of egg retrieval, a fresh sperm sample is collected from the male partner using masturbation. If there is a need sperms can be extracted from testicles or epididymis using a separate procedure. The sperm sample is then processed and the healthiest sperm is selected.
4 ) Fertilization-ICSI
Once the eggs are retrieved fertilisation process begins, where the mature eggs are placed in an incubator and are fertilized with sperm within a couple of hours. In the ICSI process, embryologist will select a single healthy sperm and directly inject into the egg. Egg fertilisation involves the observation of embryos until it reaches the blastocyst stage. Once the sperm fertilises the egg, it becomes an embryo. Monitoring of the embryo is completed in 2 to 6 days.
5 ) Blastocyst Culture
After the fertilitisation, embryo development process start. The Day 5-Day 6 stage from the fertilization is called Blastocyst.
6 ) Embryo Transfer
Once embryos are formed based on the quality and growth of the embryos, best embryos are transferred into the uterus of female partner using a small tube called a Catheter.
7 ) Pregnancy
After the embryos are transferred into the uterus our fertility experts prescribes medicine to enhance the chances implantation. After the stipulated time usually after the 14th day or 2 weeks, pregnancy tests are conducted by our fertility experts to check the result.
Here’s an example IVF journey, giving you an indication of the stages in the process and a likely number of visits to the Clinic.